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Posted: December 23rd, 2023
Which compound, A or B, is more a more potent
Concepts of Pharmaceutical ScienceProblem 3. Competition experiment.0.5 nM radioligand L* is bound to 1 ml samples of the same membrane samples used in the above experiments in the presence or absence of varying amounts of either compound A or compound B. After establishing equilibrium, the following counts were bound to the membranes (after subtracting nonspecific binding):Concentration A (nM)CPM bound with AConcentration B (nM)CPM bound with B0730010730010.05721000.05663650.1712210.1608350.3679080.3456260.5648900.5365011584011243343417153104295324455663610208581034763085883011971002808100363300961300121a). Which compound, A or B, is more a more potent displacer of the radioligand? Justify your answer why you think so.b). Plot the fractional binding on a semilog plot and estimate the IC50s for both A and B.c). Plot the results on a logit-log plot (pseudo-Hill plot) and determine the IC50s for both compounds A and B. Use these values for the next step. How close are the computed values to your estimate in part b?d). Use the Cheng-Prusoff equation and the value of Kdfor ligand L determined by the Scatchard plot in question 2 to calculate the Ki values for compounds A and B.
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