Effect of Temperature on Enzyme-catalyzed Reaction

• Yasmine Dabash

Introduction

Topic: The denaturing of enzymes.

What Citation Styles Can You Use for My Essay?

Students often ask, “Can you write my essay in APA or MLA?”—and the answer’s a big yes! Our writers are experts in every style imaginable: APA, MLA, Chicago, Harvard, you name it. Just tell us what you need, and we’ll deliver a perfectly formatted paper that matches your requirements, hassle-free.

Purpose: To further investigate catalysts and enzymes as well as their reactions to temperature change.

Problem: How does temperature affect the rate of an enzyme-catalyzed reaction?

Research

Source 1: http://www.worthington-biochem.com/introbiochem/tempeffects.html

Is It Legal to Use Your Writing Service?

Absolutely, it’s 100% legal! Our service provides sample essays and papers to guide your own work—think of it as a study tool. Used responsibly, it’s a legit way to improve your skills, understand tough topics, and boost your grades, all while staying within academic rules.

Information found: Like the majority of chemical reactions, the rate of an enzyme-catalyzed reaction raises as the temperature elevates. According to the source, “A ten degree Centigrade rise in temperature will increase the activity of most enzymes by 50 to 100%.”Many enzymes are unfavorably affected by intense heat. Because most animal enzymes quickly become denatured over 40°C, nearly all enzyme activity is accomplished under that temperature. After some time, enzymes neutralize– at even reasonable temperatures. The recommended temperature to store enzymes is 5°C or lower but even some enzymes deactivate when frozen.

Source 2: http://classroom.synonym.com/effects-temperature-enzyme-activity-biology-6049.html

Information found: Collisions between molecules increase as temperature increases. This is because of the increase in velocity and kinetic energy that is a result of increased temperature. With rapider velocities, there will be less time between molecule collisions. This results in supplementary molecules reaching activation energy, which elevates the rate of the reactions. Since the molecules are also moving quicker, collisions between enzymes and substrates also increase.

How Much Does It Cost to Get a Paper Written?

Our pricing starts at $10 per page for undergrad work, $16 for bachelor-level, and $21 for advanced stuff. Urgency and extras like top writers or plagiarism reports tweak the cost—deadlines range from 14 days to 3 hours. Order early for the best rates, and enjoy discounts on big orders: 5% off over $500, 10% over $1,000!

Identify Variables

Independent Variable: Liver temperature

Dependent Variable: The rate at which the liver rises to the surface of the hydrogen peroxide.

Controlled Variables: Environment temperature, liver origin (same individual of the same species), concentration of hydrogen peroxide solution, water composition, filter paper size, filter paper weight and density, beaker material, forceps material, Petri dish material, filter paper material, and pipette material.

Will My Use of This Service Stay Private?

Yes, totally! We lock down your info with top-notch encryption—your school, friends, no one will know. Every paper’s custom-made to blend with your style, and we check it for originality, so it’s all yours, all discreet.

Hypothesis: If enzymes are heated to 70°C then the rate of enzyme-catalyzed reactions will decrease because the denaturing of enzymes occurs after 40°C.

The Plan

Procedure:

Step 1: Put on an apron, gloves, and safety goggles. Also, make sure hair is tied up away from your face and out of the way of any experimental material.

Do You Use AI to Write Papers?

No way—our papers are 100% human-crafted. Our writers are real pros with degrees, bringing creativity and expertise AI can’t match. Every piece is original, checked for plagiarism, and tailored to your needs by a skilled human, not a machine.

Step 2: Use forceps to place raw liver in an open Petri dish. Use a pipette to put a few drops of hydrogen peroxide solution on the liver. Observe any chemical or physical changes. (Caution: Rinse off any hydrogen peroxide that comes in contact with skin, eyes, or clothing)

Step 3: Cut of a 5 cm x 5 cm piece of filter paper and absorb it with a sample of liver.

Step 4: Fill 50 a 50 mL beaker with 25 mL of 1% hydrogen peroxide solution using a graduated cylinder to measure.

Step 5: Use forceps to drop the livered filter paper to the bottom of the solution. Record using a timer how long the filter paper takes to rise to the surface. Seek assistance if needed.

Why Choose You for Research Papers?

We’re the best because our writers are degree-holding experts—Bachelor’s to Ph.D.—who nail any topic. We obsess over quality, using tools to ensure perfection, and offer free revisions to guarantee you’re thrilled with the result, even on tight deadlines.

Step 6: Now, heat 25 mL of water and a sample of liver in a 50 mL beaker to 70°C with on a hot plate. (Caution: Hot plates are called hot plates for a reason; their hot. They can burn you. Seek adult assistance if young or incapable.)

Step 7: Turn the hot plate OFF and remove the beaker from the hot plate using beaker tongs. Then remove the liver from the water using forceps and set the sample into a Petri dish.

Step 8: Repeat the previous experiment with the heated liver. Records results then analyze the data.

Step 9: Compare and contrast the data sets from each part of the experiment.

Who’s Writing My College Essays?

Our writers are top-tier—university grads, many with Master’s degrees, who’ve passed tough tests to join us. They’re ready for any essay, working with you to hit your deadlines and grading standards with ease and professionalism.

Data

Liver temperature	The time it took for the liver to reach the surface of a 1% hydrogen peroxide. (seconds)
Room Temperature Liver Are Your Papers Original? Always! We start from scratch—no copying, no AI—just pure, human-written work with solid research and citations. You can even get a plagiarism report to confirm it’s 95%+ unique, ready for worry-free submission.	5.73 seconds
70°C Liver	7.22 seconds

Results and Understandings

Can You Match Any Citation Style?

You bet! From APA to IEEE, our writers nail every style with precision. Give us your guidelines, and we’ll craft a paper that fits your academic standards perfectly, no sweat.

Graphing:

Data analysis:

When analyzing the results of the experiment, it is easy to see that there are trends in the data. The relationship between enzyme activity and temperature is inversely proportional. The higher the temperature of the enzymes, the faster they react and the less time the livered filter paper takes to rise to the surface of a 1% hydrogen peroxide solution. However, after 40°C, the denaturing of enzymes; therefore slowing down enzyme-catalyzed reactions. This is because as the denaturing of enzymes occurs they no longer fit their specific ‘lock’ to which they are ‘key’ to.

Can I Update Instructions Mid-Order?

Yep! Use our chat feature to tweak instructions or add details anytime—even after your writer’s started. They’ll adjust on the fly to keep your essay on point.

Though there was not any lack in accuracy, there was an extreme lack of precision during this experiment. Firstly, the assistant scientific hands were not at all interested in the experiment; making it exceedingly difficult to attempt to get them on task, complete the experiment, and get steps that require the cooperation of more than one individual done. It is also importance to recognize the limitation of time allotted for previously mentioned tasks to be applied; one class period. Also, it would have made all the difference in the world if the time it took for the filter paper to rise in increments of 5 mL could have been measured since it rose through 25 mL of 1% hydrogen peroxide solution. In doing this, it would be seen whether the filter paper accelerated positively or decelerated as it rose through the solution. But due to time constraints, lack of expertise, and lack of an extremely precise timing device, such an idea could not be executed. But even before trying to accomplish that out of the ballpark idea, it would have been better to allow more trials for each temperature of liver and perhaps entertain the addition of higher or lower temperatures of liver. Perhaps refrigerated liver? Even better, liver at or around 40°C.

Knowing how enzymes react to heat is vital for a number of biologists globally. Especially since biologists are required to properly handle and store enzymes. Enzymes are best stored below 5°C in order for them to not o dormant. Also, it is important for real life biologists to understand that enzymes will work faster in heat only up to a certain point. For example, in many animals, enzymes denature after they hit 40°C. Almost all enzyme activity happens below that temperature.

Conclusion

Claim:

How Do I Get an Essay Written?

Easy—place your order online, and your writer dives in. Check drafts or updates as you go, then download the final paper from your account. Pay only when you’re happy—simple and affordable!

In correlation to the hypothesis, the outcome of the experiment proved the following hypothesis: “If enzymes are heated to 70°C then the rate of enzyme-catalyzed reactions will decrease because the denaturing of enzymes occurs after 40°C.” The hypothesis gathered from the evidence pertaining to the collision theory ended up being true not only on paper but in real life experimentation and scenarios as well. Since the chemical reaction slowed when the livered filter paper was heated to 70°C, then it is easy to say with utmost certainty that the relationship between temperature and reaction time is indirectly proportional up until 40°C.

In regards to the purpose of the lab experiment, many investigations have been made that further our knowledge on how to properly handle enzymes temperature wise. Which temperature are too hot, which are not hot enough, etc. Also, from the information gathered, other intelligent assumptions can be made on the proper storage of enzymes. Since it is known that enzymes, over time, become dormant—wouldn’t it be ideal to freeze them so they retain their activity? But perhaps, that is for yet another experiment.

Evidence and Elaboration

Evidence 1:

How Fast Can You Handle Urgent Deadlines?

Super fast! Our writers can deliver a quality essay in 24 hours if you’re in a pinch. Pick your deadline—standard is 10 days, but we’ll hustle for rush jobs without skimping.

The collision theory states that as temperature rises, particles move more rigorously as collide more often. When these molecules bump into one another, reactions occur—in this example, the enzyme-catalyzed reaction is the result. The amount of collisions it takes for a full reaction to occur is the activation energy of a chemical reaction. This theory is the first piece of evidence for the increase in enzyme-catalyst activity then temperature increases.

Evidence 2:

The Induced Fit theory of enzymes accounts for the fact that the activity and substrate of an enzyme are complementary and mold to one another’s ice-cube tray, or as biologymad.com likes to say, “Mould to each other like ‘hand in glove.’” The links in substrates and enzymes bend when enzyme-substrate compounds form, deteriorating them significantly. Unlike the ‘Lock and Key’ theory of enzymes, the Induced Fit theory accounts for catalytic action and the products of chemical reactions pertaining to enzymes.

Works Cited

“Enzymes.” Biology Mad. Master Frameset, 07 Nov. 2005. Web. 07 Dec. 2014.

Can You Tackle Complex Essay Topics?

Definitely! From astrophysics to literary theory, our advanced-degree writers thrive on tough topics. They’ll research deeply and deliver a clear, sharp paper that meets your level—high school to Ph.D.

“Introduction to Enzymes: Temperature Effects.” Worthington Biochemistry. Worthington Biochemical Corporation, n.d. Web. 07 Dec. 2014.

Santhosh, Lakshmi. “The Effects of Temperature on Enzyme Activity.” Synonym. Demand Media, 14 Aug. 2013. Web. 05 Dec. 2014.

Tags: Buy essay USA, Custom Dissertation Writing Services for PhD Students, UK - Cheap Essay Writing Service, Write My Essay Fast - Plagiarism-Free AI Writing Tool