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Posted: January 1st, 1970
Experiment 3- Blood II
Rezan Nehir MavioÄŸlu, Tara McDermott, Yasemin Barış, Dogan ÇolakoÄŸlu
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Introduction
In this lab, we took our own blood samples and observed the presence and characteristics of our white blood cells, and our specific blood type. Through this experiment, we determined the number of white blood cells present in 1mm3 of blood, the different types present, and our unique blood types.
Before performing the experiment, we knew (collectively) white blood cells protect the body from foreign invaders and pathogens. The body’s defense mechanism via white blood cells is either innate (reacts with low specificity to foreign pathogens) or acquired (antigen specific response). In this particular lab, we identified eosinophils (granulocyte/phagocyte), neutrophil (granulocyte, phagocyte), macrophages and monocytes (phagocytes), and lymphocytes (cytotoxic cells) within our blood. White blood cells are classified by staining procedures (we used Wright’s stain to identify WBC’s based on intracellular pH) or by the presence of cytoplasmic granules. Granulocytes contain cytoplasmic granules, phagocytes ingest foreign invaders, and lymphocytes comprise part of the acquired immune system.
Blood type is determined by the presence of extracellular antigenic “markers” on the surface of a cell membrane. The blood types are as follows:
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A: only A antigens of red cells
B: B antigens extracellular
AB: both A and B antigens extracellular
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O: neither A or B on surface
Individuals with A carry antibodies for B, type B carries antibodies for A, and type AB carries no antibodies to A or B. Type O individuals carry antibodies for both A and B.
Additionally, there is a third factor called “Rh factor”, which is either present (+) or absent (-) on the surface of a cell. Rh + individuals can receive both + and – blood, but Rh – individuals can only receive Rh – blood. Thus, Type O- is referred to as the universal red cell “donor”.
We determined our blood type by adding anti A, anti B, and anti D antibody solutions to each of our blood samples and observing the coagulation. For example, if a blood sample coagulates where anti A is added, and where anti Rh is added, then the individual’s blood type is A+.
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Materials and Methods
Materials- Experiment 1: Total White Blood Cell counts
Blood (taken from experimenters)
Lancet
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Microscope
Hematocytometer (for samples)
Turck’s solution (for staining white blood cells’ nuclei)
Method: We lanced our finger, taking about 5 ul of blood and mixing it with 95 ul of Turck’s solution to stain the nuclei of our cells- so as to only identify white blood cells. We took 20 ul of this 1:20 dilution and filled the hematocytometer. Using a lowe power objective, we counted all visible nuclei within 18 squares of the counting chamber.
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Materials- Experiment 2: Differential White Blood Cell Types
Lancet
Blood
Clean Glass slides
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Wright’s stain (to identify white blood cell types based on pH)
Microscope
Method: Taking another drop of blood, we added it to a glass slide and covered it, allowing it to dry. We added Wright’s stain and incubated for about 2 minutes, adding additional stain to keep the sample from drying out. After rinsing the slide with water and allowing it to dry, we observed the sample under a microscope. Based on pH differences, presence of intracellular granules, and shape of nuclei, we counted and classified 200 white blood cells.
Materials- Experiment 3: Blood Types
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Blood
Lancet
Glass slide
Anti A, Anti B, and Anti Rh (D) serum
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Method: We placed three drops of blood on a glass slide, and added the anti solutions to each. After smearing and allowing time for coagulation, we observed the samples to determine blood type.
Results
Total WBC counts
White blood cell counts were obtained from the number of WBCs in 16 squares of hemocytometer. 16 squares have a volume of 0.1 mm3 and the blood samples were diluted 1:20. Therefore, total WBC counts in 1 mm3 was calculated by multiplying the observed number by 200. Please see Table 1 for WBC counts per mm3 for each group member.
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Histogram of WBC counts of a sample of 40 males and 40 females can be seen in Figure 1. Independent samples t-test revealed that there were no gender differences in mean WBC counts (p=0.86). Descriptives for WBC counts can be seen in Table 2. According to the table, DoÄŸan and Yasemin are in the last percentile, and Tara and Nehir exceed the maximum value.
Figure 1: Histogram of WBC counts of sample group, with normal curve
Table 1: Observations and measurements for each group member
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Gender |
WBC/mm3 |
Blood Type |
Neutrophil How Fast Can You Handle Urgent Deadlines?Super fast! Our writers can deliver a quality essay in 24 hours if you’re in a pinch. Pick your deadline—standard is 10 days, but we’ll hustle for rush jobs without skimping. |
Eosinophil |
Basophil |
Lymphocyte |
Monocyte&Macrophage |
|
DoÄŸan Can You Tackle Complex Essay Topics?Definitely! From astrophysics to literary theory, our advanced-degree writers thrive on tough topics. They’ll research deeply and deliver a clear, sharp paper that meets your level—high school to Ph.D. |
M |
9600 |
A Rh – |
4 (44.4 %) |
0 How Do You Meet My Professor’s Standards?We tailor your paper to your rubric—structure, tone, everything. Our writers decode academic expectations, and editors polish it to perfection, ensuring it’s grade-ready. |
0 |
4 (44.4 %) |
1 (11.1 %) |
Nehir What’s Your Editing Process?Upload your draft, tell us your goals, and our editors will refine it—boosting arguments, fixing errors, and keeping your voice. You’ll get a polished paper that’s ready to shine. |
F |
14400 |
0 Rh + |
2 (5.9%) |
2 (5.9 %) Can You Suggest Paper Topics?Sure! Need ideas? We’ll pitch topics based on your subject and interests—catchy and doable. Pick one, and we’ll run with it, or tweak it together. |
0 |
18 (52.9 %) |
12 (35.3 %) |
Tara |
F |
12800 |
0 Rh + Do You Offer Rush Revisions?Yes! If you need quick edits, our team can turn it around fast—hours, not days—tightening up your paper for last-minute perfection. |
2 (16.7 %) |
2 (16.7 %) |
0 |
2 (16.7 %) |
6 (50 %) |
Yasemin Can You Provide Outlines First?Absolutely! We’ll draft an outline based on your topic so you can approve the plan before we write—keeps everything aligned from the start. |
F |
10400 |
0 Rh + |
5 (62.5 %) |
0 |
1(12.5%) |
2 (25%) |
0 Can You Include Data Analysis?You bet! Need stats or charts? Our writers can crunch numbers and craft visuals, making your paper both sharp and professional. |
Table 2: Statistics for WBC counts/ul |
||
N |
Valid |
80 |
Missing |
0 |
|
Mean |
7202,1375 |
|
Minimum How Do You Handle Long-Term Projects?We break it down—delivering each part on time with consistent quality. From proposals to final drafts, we’re with you all the way. |
2395,00 |
|
Maximum |
11256,00 |
|
Percentiles |
25 Can You Write for Global Standards?Yep! Whether it’s UK, US, or Australian rules, we adapt your paper to fit your institution’s style and expectations perfectly. |
5655,0000 |
50 |
6987,5000 |
|
75 |
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Differential white blood cell counts
Please see Table I for differential WBC counts in percentages for each group member. DoÄŸan and Yasemin has neutrophil as dominant WBC, whereas Nehir has lymphocyte and Tara has monocyte & macrophage.
Blood Types
For Nehir, Yasemin and Tara, only anti D serum formed aggregate. For DoÄŸan, only anti A serum formed aggregate. Please see Table I for blood groups of each group member. Percentages and frequencies for each blood type can be seen in Table 3. Distribution of blood types according to the sample data can be seen in Figure 2.
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Table 3: Frequencies and percentages of each blood type
Frequency |
Percent |
||
A Rh + |
30 |
37,5 |
|
A Rh – |
4 |
5,0 |
|
B Rh + |
11 |
13,8 |
|
B Rh – |
2 |
2,5 |
|
AB Rh + |
6 |
7,5 |
|
AB Rh – |
1 |
1,3 |
|
O Rh + |
22 |
27,5 |
|
O Rh – |
4 |
5,0 |
|
Total |
80 |
100,0 Figure 2: Distribution of blood types Discussion The aim of this experiment was to measure the WBC counts, observe the different types of WBCs and see the distribution among them, and determine our blood type by coagulation reaction by anti A-B and D serums. The first part of the experiment was measuring WBC counts. Only DoÄŸan and Yasemin were in the normal range according to sample data provided. According to NIH, normal WBC range is from 4500 to 10000. Therefore, Yasemin is just slightly above the range, and Tara and Nehir were definetely above the range. This result may be due to anemia, infections or an inflammatory disease. Drugs, some diseases, and radiation may also affect WBC count. The second part of the experiment was to observe different cell types. Neutrophil was dominant for DoÄŸan and Yasemin, lymphocite was dominant for Nehir and monocytes & macrophages was dominant for Tara. Neutrophil should be the dominant cell type in blood, so we can say that DoÄŸan and Yasemin were healthy during the experiment day. High lymphocyte number is an indication of hepatitis, mononucleosis, leukemia, viral infections, and bacterial infections. Nehir might have caught flu (we hope). An increased number of monocytes indicate inflammatory disease, leukemia, or viral infections. Tara may have viral infections. Apart from that, none of us could not count 200 cells as we supposed to do. Therefore, these results may be misleading. The last part of the experiment was to determine the blood types. DoÄŸan has A Rh -, and the rest of the group has 0 Rh + type of blood. This result does not fit the distribution shown, with A Rh + dominancy. (see Figure 2 and Table 3). However, 4 people is not enough to prepare a statistics and obtain meaningful results. Ethnicity seems to be a major component of blood type variation among different countries. Pure ethnic groups might have specific blood types due to genetics and geography. References 1. Lab manual 2. Silverthorn, Dee U., et al. Human physiology : an integrated approach. Boston: Pearson Education, 2013. Print. 3. Hardy Diagnostics. “Wrights Stain.” Hugo. Hardy Diagnostics, n.d. Web. https://catalog.hardydiagnostics.com/cp_prod/content/hugo/OneStepWrightsStain.htm. 4. Red Cross. “Blood Types.” American Red Cross. American Red Cross, n.d. Web. http://www.redcrossblood.org/learn-about-blood/blood-types. 5. Blood Book. “Racial & EthnicDistribution of ABO Blood Types” http://www.bloodbook.com/world-abo.html. 6. National Institues of Health. MedlinePlus. “WBC count.” http://www.nlm.nih.gov/medlineplus/ency/article/003657.htm 7.National Institues of Health. MedlinePlus. “Blood differential.” http://www.nlm.nih.gov/medlineplus/ency/article/003643.htm Tags: Affordable Assignment Assistance, Custom Essay Writing, Free Essay Samples, Online Homework HelpWhy Choose Essay Bishops?You Want The Best Grades and That’s What We Deliver Top Essay WritersOur top essay writers are handpicked for their degree qualification, talent and freelance know-how. Each one brings deep expertise in their chosen subjects and a solid track record in academic writing. Affordable PricesWe offer the lowest possible pricing for each research paper while still providing the best writers;no compromise on quality. Our costs are fair and reasonable to college students compared to other custom writing services. 100% Plagiarism-FreeYou’ll never get a paper from us with plagiarism or that robotic AI feel. We carefully research, write, cite and check every final draft before sending it your way. |