Capillary Zone Electrophoresis Experiment

Abstract:

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 The goal of the experiment was to determine the effect of pH on Electroosmotic Flow (EOF) and quantification of amino acids in cutler Amino Pump sample by using capillary electrophoresis (CE). For module 1, DMFA was used in three different buffer pH 5.0, 7.0 and 9.5. DMFA was used because able to retain neutral compound despite change in pH value. Increase in pH made the elution time of DMFA become shorter. For module 2, the quantification of leucine and aspartic acid in Cutler sample was determine by using standard addition technique. The different in elution times between leucine and aspartic acid peaks due to charge difference. OPA-BME was used to derivatize the amino acids to make the amino acid become detectable under UV-Vis. The initial concentration of leucine is 0.55 mM and aspartic acid is 2.33 mM in 0.1828 g of Cutler Amino Pump sample. The mass of amino acid per gram of Cutler is 0.02 for leucine and 0.085 for aspartic acid.