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Posted: July 9th, 2020
Bioinformatics: Clustal W and Consurf, science homework help
Question description
a) What do
the “*” and ":" indicate on the MSA alignment?
b) Why does the
secondary structure prediction come back with a higher percentage of
random coil then actually exists in the protein? Is this prediction
accurate and/or realistic? Why or why not? Please explainc) What is a conserved sequence motif? d)
Is
there a relationship between the sequence conservation and the overall 3-D
structure? e) The
exercise looked at the multiple sequence alignment of a protein. How
would you use MSA on a nucleic acid (such as a tRNA) and what information might
you obtain from that exercise? Will you always look simply for conservation or
are there other factors that might come into play? To answer this question,
think about the structure of tRNA. If you don’t know what tRNA looks like, work
through this tRNA tutorial before answering the question.
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